動物実験施設を利用した各講座の2005年度研究業績


秋田大学医学部動物実験施設を使用し得られた研究成果のうち2005年度に公表された論文．

原著論文

構造機能医学講座

形態解析学分野・器官構造学分野（旧解剖学第一講座）
  1. Zhou, M., Tanaka, O., Sekiguchi, M., He, H-J., Yasuoka, Y., Itoh, H., Kawahara, K. and Abe, H. (2005) ATP-sensitive K+-channel subunits on the mitochondria and endoplasmic reticulum of rat cardiomyocytes. J. Histochem. Cytochem., 53, 1491-1500.

構造機能医学講座

細胞生物学分野（旧解剖学第二講座）
  1. Saito, M., Satoh, S., Kojima, N., Tada, H., Sato, M., Suzuki, A., Tsuchiya, N., Suzuki, T., Senoo, H. and Habuchi, T. (2005) Effects of phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury. Arch. Histol. Cytol., 68, 41-49.
  2. Sato, M., Sato, T., Kojima, N., Imai, K., Higashi, N., Wang, D.-R. and Senoo, H, (2004) Three-dimensional structure of extracellular matrix regulates gene expression in cultured stellate cells to induce process elongation. Comp. Hepatol., 3(Suppl 1),S4.
  3. Higashi, N., Sato, M., Kojima, N., Irie, T., Kawamura, K., Mabuchi, A. and Senoo, H. (2005) Vitamin A storage in hepatic stellate cells in the regenerating rat liver-with special reference to zonal heterogeneity-. Anat. Rec., 286A, 899-907.

分子機能学分野・代謝機能学分野（生化学第一講座）
  1. Kanzaki-Kato, N., Tamakoshi, T., Fu, Y. et al. (2005) Roles of forkhead transcription factor Foxc2 (MFH-1) and endothelin receptor A in cardiovascular morphogenesis. Cardiovasc. Res.,　65, 711-718.
  2. Ise, N., Sato, T., Yasui, O., Watanabe, G., Koyama, K., Terada, K., Sugiyama, T. and Yamamoto, Y. (2004) Enhanced proliferation of hepatic progenitor cells in rats after portal branch occlusion. Liver Transpl., 10, 748-754.
  3. Kameda, T. and Sugiyama, T. (2005) Application of genetically modified feeder cells for culture of keratinocytes. Methods Mol. Biol., 289, 29-38.
  4. Deng, W. G., Fu, Y., Li, Y. L. and Sugiyama, T. (2004) Potential role of p53 mutation in chemical hepatocarcinogenesis of rats. World J. Gastroenterol., 10, 46-52.
  5. Ueno, Y, Nagai, H, Watanabe, G, Ishikawa, K, Yoshikawa, K, Koizumi, Y., Kameda, T. and Sugiyama, T. (2005) Transplantation of rat hepatic stem-like (HSL) cells with collagen matrices. Hepatol. Res. , 33, 277-284.
  6. Guo, N., Liu, Y., Masuda, Y., Kawagoe, M., Ueno, Y., Kameda, T. and Sugiyama, T. (2005)　Repeated immunization induces the increase in fucose content on antigen-specific IgG N-linked oligosaccharides. Clin. Biochem., 38,149-153.
  7. Kawagoe, M., Hirasawa, F., Wang, S.-C., Liu, Y., Ueno, Y. and Sugiyama, T. (2005) Orally administrated rare earth element cerium induces metallothionein synthesis and increases glutathione in the mouse liver. Life Sci., 77, 922-937.
  8. Hirasawa, F., Kawagoe, M., Arany, S., Koizumi, Y., Ueno, Y. and Sugiyama, T. (2005) Styrene monomer primarily induces CYP2B1 mRNA in the rat liver. Xenobiotica, 35, 1089-1099.

分子医科学分野（生化学第二講座）
  1. Watanabe, S., Horie, Y. and Suzuki, A. (2005)Hepatocyte-specific Pten-deficient mice as a novel model for nonalcoholic steatohepatitis and hepatocellular carcinoma. Hepatol. Res., 33, 161-166.
  2. Hamada, K., Sasaki, T., Koni, P.A. et al. (2005)The PTEN/PI3K pathway governs normal vascular development and tumor angiogenesis. Gene. Dev., 19, 2054-2065.
  3. Sasaki, J., Sasaki, T., Yamazaki, M. et al.(2005) Regulation of anaphylactic responses by phosphatidylinositol phosphate kinase type I alpha. J.Exp.Med., 201, 859-870.
  4. 片岡　英, 堀江泰夫, 佐藤　亘ら(2005)DNAマイクロアレイを用いた肝細胞特異的Pten欠損マウスの肝臓における遺伝子発現の検討　―非アルコール性脂肪性肝炎の発症に関わる新たな遺伝子の検索を目的として―.秋田医学32, 41-51.

機能制御医学講座

細胞制御学分野（生理学第一講座）
  1. Wang, Y., Yamada, K., Tanaka, Y., Ishikawa, K. and Inagaki, N. (2005) Expression of BCA2 protein in human vestibular schwannoma and peripheral nerve. J. Neurol. Sci., 232, 59-63.
  2. Ban, N., Sasaki, M., Sakai, H., Ueda, K. and Inagaki, N. (2005) Cloning of ABCA17, a novel, rodent sperm-specific ATP-binding cassette (ABC) transporter that regulates intracellular lipid metabolism. Biochem. J., 389, 577-585.

器官制御学分野・統合制御学分野（生理学第二講座）
  1. Shinozaki, S., Saito, M. and Kawatani, M.(2005) Loxoprofen inhibits facilitated micturition reflex induced by acetic acid urinary bladder infusion of the rat. Biomedical Res., 26, 29-33.
  2. Saito, M., Kawatani ,M., Kinoshita, Y., Satoh ,K. and Miyagawa, I. (2005)Effectiveness of an anti-inflammatory drug,loxoprofen, for patients with nocturia. Int. J. Urology , 12, 779-782.

情報制御学分野・実験治療学分野（薬理学講座）
  1. Harada, K., Xu, F., Ono, K. Iijima,T. and Koizumi, A. (2005) Effect of PFOS and PFOA on L-type Ca2+ current in guinea pig ventricular myocytes. Biochem. Biophys. Res. Commun., 329, 487-494.

病理病態医学講座

分子病態学分野・腫瘍病態学分野（病理学第一講座）
  1. Nishikawa, Y., Doi, Y., Watanabe, H., Tokairin, H., Omori, Y., Su, M., Yoshioka, T. and Enomoto, K. (2005) Transdifferentiation of mature rat hepatocytes into bile duct-like cells in vitro. Am. J. Pathol., 166, 1077-1088.

器官病態学分野（病理学第二講座）
  1. Sho, E., Sho, M., Nanjo, H., Kawamura, K., Masuda, H. and Dalman, R.L. (2005) Comparison of cell-type-specific vs transmural aortic gene expression in experimental aneurysms. J. Vas. Surg., 41, 844-852.
  2. Yamauchi, M., Takahashi, M., Kobayashi, M., Sho, E., Nanjo, H., Kawamura, K. and Masuda, H. (2005) Normalization of high-flow or removal of flow cannot stop high-flow induced endothelial proliferation. Biomed. Res., 26, 21-28.
  3. Nanjo, H., Sho, E., Komatsu, M., Sho, M., Zarins, C.K., Masuda, H. (2005) Intermittent short-duration exposure to low wall shear stress induces intimal thickening in arteries exposed to chronic high shear stress. Exp. Mol. Pathol. 2005; available online 15 June.
  4. Matsumoto, T., Okumura, E., Shirono, T., Sho, E., Masuda, H. and Sato, M. (2005) Flow-induced changes in dimensions and mechanical properties of rabbit common carotid arteries. JSME Int. J. Series C, 48, 477-483.
  5. Yamamoto, K., Sokabe, T., Matsumoto, T. et al. (2005) Impaired flow-dependent control of vascular tone and remodeling in P2X4-deficient mice. Nature Medicine, 12, 133 -137.
  6. Higashi, N., Sato, M., Kojima, N., Irie, T., Kawamura, K., Mabuchi, A. and Senoo, H. (2005) Vitamin A storage in hepatic stellate cells in the regenerating rat liver : with special reference to zonal heterogeneity. Anat. Rec. Part A. 286A, 899-907.

社会環境医学講座

感染制御学分野（微生物学講座）
  1. Sasaki, J., Sasaki, T., Yamazaki, M. et al. (2005)Regulation of anaphylactic responses by phosphatidylinositol phosphate kinase type I alpha. J. Exp.Med.,201, 859-870.

生体防御
  1. Tada, H., Aiba, S., Shibata, K., Ohteki, T. and Takada, H. (2005) Synergistic effects of Nod1 and Nod2 agonists with Toll-like receptor agonists on human dendritic cells to generate interleukin-12 and T helper type 1 cells. Infect. Immun., 73, 7967-7976.

内科学講座

消化器内科学分野・神経内科学分野 （内科学第一講座）
  1. Otaka,M. and Watanabe,S.(2005)Expression and Function of Stress(Heat Shock) Proteins in Digestive Organs.　Japanese Journal of Hyperthermic Oncology., 21,81-93.
  2. Mikami,K.,Goto,T.,Miura,K. et al. (2005) Gabexate mesilate, a synthetic protease inhibitor, attenuates carbon tetrachloride-induced liver injury in rats. J.Gastroenterol.,40,260-265.
  3. Odashima, M., Giorgos, B., Jesus R-N. et al.(2005) Activation of A2A Adenosine Receptor Attenuates Intestinal Inflammation in Animal Models of Inflammatory Bowel Disease.Gastroenterology.,129,26-33.
  4. Odashima,M.,Otaka,M.,Jin,M. et al.(2005) Rolipram,a Specific Type ?｢ Phosphodiesterase Inhibitor,Ameliorates Aspirin-Induced Gastric Mucosal Injury in Rats. Digestive Diseases and Sciences.,50,1097-1102.
  5. Odashima,M.,Otaka,M.,Jin,M. et al. (2005) Selective adenosine A2A receptor agonist,ATL-146e,attenuates stress-induced gastric lesions in rats. Journal of Gastroenterology and Hepatology,20,275-280.
  6. Odashima,M.,Otaka,M.,Jin,M. et al.(2005) Selective A2A adenosine agonist ATL-146e attenuates acute lethal liver injury in mice. J. Gastroenterol.,40,526-529.
  7. Komatsu,K.,F.Gregory,Buchanan.,Sharada,Katkuri.,Jason.D.Morrow.,Inoue,H.,Otaka,M.,Watanabe,S. and Rymond N.Dubois.(2005)Oncogenic Potential of MEK1 in Rat Intestinal Epithelial Cells Is Medeated via Cyclooxyg enase-2. Gastroenterology, 129,577-590.
  8. Matsuhashi T, Otaka M, Odashima M. et al.(2005)Specific type IV phosphodiesterase inhibitor ameliorates thioacetamide-induced liver injury in rats. J. Gastroenterol. Hepatol.,20, 135-40.
  9. Segawa,D.,Miura,K.,Goto,T. et al. (2005) Distribution and isoforms of epimorphin in carbon tetrachloride-induced acute liver injury in mice. J. Gastroenterol. Hepatol.,20,1769-1780.
  10. Takeuchi,S.,Goto,T.,Mikami,K.et al.(2005) Genipin prevents fulminant hepatic failure resulting in reducation of lethality through the suppression of TNF-α production. Hepatology Research,33,298-305.
  11. 大高道郎,小西永晃,小田嶋　傑ら（2005）ストレスが血清,胃粘膜のレプチン発現に及ぼす影響−ラットを用いた検討−．消化器心身医学12,7-11
  12. 大高道郎，小田嶋　傑，神　万里夫，和田　勲，渡辺純夫（2005）膵における熱ショック蛋白質の発現と機能．G.I.Research,13,30-35.
  13. 片岡　英，堀江泰夫，佐藤　亘ら（2005）DNAマイクロアレイを用いた肝細胞特異的Pten欠損マウスの肝臓における遺伝子発現の検討　―　非アルコール性脂肪性肝炎の発症に関わる新たな遺伝子の検索を目的として　―．秋田医学32,41-51.
  14. Miura,K.,Segawa,D.,Yoshino,R.,Goto,T.,Ohshima,S., Mikami,K.,Yoneyama,K., Watanabe,D.,Kataoka,E. and Watanabe,S.(2005) In volvement of epimorphin in remodeling of sinusoid in regenerative and injured liver. Microcirculation annual.,21,57-58.

呼吸器内科学分野・循環器内科学分野　 （内科学第二講座）
  1. Ohba, T.,Watanabe, H.,Murakami, M.,Takahashi, Y,. and Ito, H. (2005) Increased 　　expreesion of transient receptor potential C1 in rats with hypertensive hypertrophy.　　Akita J. Med., 32,201-207

内分泌・代謝・老年医学分野（老年医学講座）
  1. Fujita, H., Fujishima, H., Koshimura, J., Hosoba, M., Yoshioka, N., Shimotomai, T., Morii, T., Narita, T., Kakei, M. and Ito, S. (2005) Effects of antidiabetic treatment with metformin and insulin on serum and adipose tissue adiponectin levels in db/db mice. Endc. J.,52, 427-433.

外科学講座

呼吸器外科学分野（外科学第二講座）
  1. Saito，S．，Ogawa，J． and Minamiya，Y．（2005）Pulmonary reexpansion causes xanthine oxidase-induced apoptosis in rat lung．Am. J. Physiol. Lung. Cell Mol.　Physiol.，289， 400-406．

脳神経外科学分野（脳神経外科学講座）
  1. Sugawara, T.， Kinouchi, H.， Oda, M.， Shoji, H.， Omae, T. and Mizoi, K.（2005）Candesartan reduces superoxide production after global cerebral ischemia． Neuroreport,16, 325-328.
  2. 小田正哉，木内博之，呉　繁夫，菅原　卓，山口　卓，大前智也，佐藤健一，松原洋一，溝井和夫(2005)局所脳虚血後の神経細胞障害におけるグリシンの役割について―高グリシン症マウスを用いての検討―．秋田医学32, 53-61.

生殖発達医学講座

産婦人科学分野（産科婦人科学講座）
  1. Kawamura, K., Fukuda, J., Shimizu, Y., Kodama, H. and Tanaka, T.(2005)Survivin contributes to the anti-apoptotic activities of transforminggrowth factor a in mouse blastocysts through phosphatidylinositol-3’-kinase pathway. Biol. Reprod.,73,1094-1101.
  2. Kumazawa,Y.,Kawamura,K.,Sato,T.,Sato,N.,Konishi,Y.,Shimizu,Y.,Fukuda,J.,Kodama, H. and Tanaka,T.(2005)HCG up-regulates survivin mRNA in human granulosa cells. Mol. Hum. Reprod., 11,161-166.
  3. Honda,Y.,Tanikawa,H.,Fukuda,J.,Kawamura,K.,Sato,N.,Sato,T.,Shimizu,Y.,Kodama,H. and Tanaka, T.(2005)Expression of Smac/DIABLO in mouse preimplantation embryos and its correlation to apoptosis and fragmentation. Mol. Hum. Reprod.,11,183-188.

泌尿器科学分野（泌尿器科学講座）
  1. Minagawa, S., Ohyama, C., Hatakeyama, S., Tsuchiya, N., Kato, T. and Habuchi, T. (2005) Activation of natural killer T cells by alpha-galactosylceramide mediates clearance of bacteria in murine urinary tract infection. J. Urol., 173, 2171-2174.
  2. Saito, M., Satoh, S., Kojima N, Tada, H., Sato, M., Suzuki, T., Senoo, H. and Habuchi, T. (2005) Effects of a phenolic compound, resveratrol, on the renal function and costimulatory adhesion molecule CD86 expression in rat kidneys with ischemia/reperfusion injury. Archives Histology and Cytology, 68, 41-49

統合医学講座

麻酔科学・蘇生学分野（麻酔学講座）
  1. Kimura, T., Sato, M., Nishikawa, T., Tanaka, M., Tobe, Y. and Masaki, Y. (2005) Neuroprotective effect of mivazerol, an alpha 2-agonist, after transient forebrain ischemia in rats. Acta Anaesthesiol. Scand., 49, 1117-1123.
  2. Nagata, H., Horiguchi, T., Enzan, K., Nishikawa, T. and Suzuki, K. (2005) Inhibition of poly (ADP-ribose) synthetase improves pulmonary arterial endothelium-dependent relaxation after ischemic-reperfusion injury of splanchnic artery in rats. Anesth. Analg., 101, 1799-1804.

診療科

歯科口腔外科
  1. Fukuda, M., Miyamoto, Y., Ohnuki, T., Nagai, H, Takechi, M., Matsuya, S. and Ishikawa, K. (2005) Application of carbonate apatite to bone reconstruction: fabrication and histological evaluation of low crystalline carbonate apatite. Archives of BioCeramics Research 5 (The 5th Asian BioCeramics Symposium proceedings), 75-78.

中央診療施設等

中央手術部
  1. Nagata, H., Horiguchi, T., Enzan, K., Nishikawa, T and SuzukI, K. (2005) Inhibition of Poly (ADP-ribose) Synthetase improves pulmonary arterial endothelium-dependent relaxation after ischemic-reperfusion injury of splanchnic artery in rats. Anesth. Analg., 101, 1799-1804.

病理部
  1. Nanjo, H., Sho, E., Komatsu, M., Sho, M., Zarins, C.K. and Masuda, H.(2005) Intermittent short-duration exposure to low wall shear stress induces intimal thickening in arteries exposed to chronic high shear stress. Exp. Mol. Pathol., available online 15 June.
  2. Yamauchi, M., Takahashi, M., Kobayashi, M., Sho, E., Nanjo, H., Kawamura, K. and Masuda, H. (2005) Normalization of high-flow or removal of flow cannot stop high-flow induced endothelial proliferation. Biomed. res., 26, 21-28.

バイオサイエンス教育・研究センター　

動物実験部門（動物実験施設）
  1. 河原崎哲、夏井美幸、松田幸久、佐々木雄彦、仲野徹、鈴木聡. (2005) PTEN変異マウスによる疾患モデルマウス、 分子細胞治療, 4(2), 63-68
  2. Somboonthum P, Ohta H, Yamada S. et. al.(2005) cAMP-responsive element in TATA-less core promoter is essential for haploid-specific gene expression in mouse testis. Nucleic Acids Res. 33(10): 3401-3411.
  3. Ihara M, Kinoshita A, Yamada S. et. al.(2005) Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa. Developmental Cell 8, 1-10.
  4. Oka SI, Liu W, Masutani H, Hirata H, Shinkai Y, Yamada S, Yoshida T, Nakamura H, Yodoi J. (2006) Impaired fatty acid utilization in thioredoxin binding protein-2 (TBP-2)-deficient mice: a unique animal model of Reye syndrome. FASEB J.
  5. Yajima N, Yamada S, Morisaki T, Toyokuni S, Yonehara S, Sakamaki K, (2005) Partial correction of abnormal cardiac development in caspase-8-deficient mice by cardiomyocyte expression of p 35. Transgenic Res. Oct;14(5):593-604.