動物実験施設を利用した各講座の2003年度研究業績


秋田大学医学部動物実験施設を使用し得られた研究成果のうち2003年度に公表された論文．

原著論文

構造機能医学講座

細胞生物学分野（旧解剖学第二講座）
  1. Higashi, N. and Senoo, H. (2003) Distribution of vitamin A-storing lipid droplets in hepatic stellate cells in liver lobules-A comparative study-. Anat. Rec., 271A, 240-248.
  2. Suzuki, A., Itami, S., Ohishi, M, et al. (2003) Keratinocyte-specific pten deficiency results in epidermal hyperplasia, accelerated hair follicle morphogenesis and tumor formation. Cancer Res., 63, 674-681
  3. Miura, K., Nagai, H., Ueno, Y. et al. (2003) Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. Biochem. Biophys. Res. Commun., 311, 415-423.

分子機能学分野・代謝機能学分野（生化学第一講座）
  1. Nakata, A., Kameda, T., Nagai, H., Ikegami, K., Duan, Y., Terada, K. and Sugiyama, T. (2003) Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. Biochem. Biophys. Res. Commun., 308, 834-839.
  2. Kameda, T., Nakata, A., Mizutani, T., Terada, K., Iba, H. and Sugiyama, T. (2003) Analysis of the cellular heterogeneity in the basal layer of mouse ear epidermis: an approach from partial decomposition in vitro and retroviral cell marking in vivo. Exp. Cell Res., 283, 167-183.
  3. Masuda, Y., Suzuki, M., Takemura, T., Sugawara, J., Guo, N., Liu, Y., Kawarada, Y., Shimizu, T. and Sugiyama, T. (2003) Pharmacological mechanism in slip-down behavior of mice. Tohoku J. Exp. Med., 201, 23-27.
  4. Miura, K., Nagai, H., Ueno, Y. et al. (2003) Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. Biochem. Biophys. Res. Commun., 311, 415-423.
  5. Fu, Y., Deng, W., Kawarada, Y., Kawagoe, M., Ma, Y.-Z., Li, X., Guo, N., Kameda, T., Terada, K. and Sugiyama, T. (2003) Mutation and expression of the p53 gene during chemical hepatocarcinogenesis in F344 rats. Biochim. Biophys. Acta, 1628, 40-49.
  6. Hatakeyama, S., Ma, Y.-Z., Miura, N., Abe, S., Kameda, T., Sakamoto, K. and Sugiyama, T. (2003) Production of monoclonal antibodies recognizing human hair follicle keratinocytes. Hybrid Hybridomics, 22, 127-130.
  7. Masuda, Y., Ohnuma, S., Kawagoe, M. and Sugiyama, T. (2003) A glycoside of Nicotina tabacum affects mouse dopaminergic behavior. Methods Find. Exp. Clin. Pharmacol., 25, 41-43.
  8. Guo, N., Liu ,Y., Masuda, Y., Kawarada, Y., Kawagoe, M., Yoshikawa, K., Kameda, T. and Sugiyama, T. (2003) The role of IgG carbohydrates in anaphylaxis: a possible way for antigen-specific anaphylaxis treatment by deglycosylated IgG. Biomed. Res., 24, 291-297.

分子医科学分野（生化学第二講座）

  1. Suzuki, A., Itami, S., Ohishi, M. et al. (2003) Keratinocyte-specific Pten deficiency results in epidermal hyperplasia, accelerated hair follicle morphogenesis and tumor formation. Cancer Res., 63(3), 674-681.

機能制御医学講座

細胞制御学分野（生理学第一講座）
  1. Tanaka, Y., Yamada, K., Zhou, C.J., Ban, N., Shioda, S. and Inagaki, N. (2003) Temporal and spatial profiles of ABCA2-expressing oligodendrocytes in the developing rat brain. J. Comp. Neurol., 455, 353-367.

器官制御学分野・統合制御学分野（生理学第二講座）
  1. Ishida, F., Sato, T., Imaizumi, M., Funayama, N., Ikegami, K., Nimura, T. and Kawatani, M.（2003） MK-801 inhibits the micturition reflex in chronic bladder irritation caused by crystalluria in the rat.　Autonomic Neuroscience: Basic & Clinical, 105, 1-7.
  2. Miura, A., Kawatani, M.and de Groat, W.C. (2003) Excitatory synaptic currents in lumbosacral parasympathetic preganglionic neurons evoked by stimulation of the dorsal commissure. J. Neurophysiol., 89, 382-389.
  3. Kimura, H., Kawatani, M., Ito, E. and Ishikawa, K.(2003) Effects of pituitary adenylate cyclase-activating polypeptide on facial nerve recovery in the guinea pig. The Larygoscope, 113, 1000-1006.
  4. 河谷正仁,　石濱寛子 （2003） 排尿障害とα１受容体−α１受容体の役割 −　脳21, 6， 43-46.
  5. 三浦　章，河谷正仁（2003）　排尿障害とα１受容体−腰仙部脊髄副交感神経節前ニューロン活動へのα１ブロッカーの及ぼす影響−．脳21, 6， 56-61.

情報制御学分野・実験治療学分野（薬理学講座）
  1. Murakami, M., Xu, F., Miyoshi, I., Satoh, E., Ono, K. and Iijima, T. (2003) Identification and characterization of the murine TRPM4 channel. Biochemical and Biophysical research comm. , 522-528.

病理病態医学講座

器官病態学分野（病理学第二講座）
  1. Sho, E., Sho, M., Singh, T.M., Nanjo, H., Komatsu, M., Xu, C., Masuda, H.and Zarins, C.K. (2002) Arterial enlargement in response to high flow requires early expression of matrix metalloproteinases to degrade extracellular matrix. Experimental and Molecular Pathology, 73, 142-153.
  2. Sho, M., Sho, E., Singh, T.M., Komatsu, M., Sugita, A., Xu, C., Nanjo, H., Zarins, CK.and Masuda, H. (2002) Subnormal shear stress-induced intimal thickening requires medial smooth muscle cell proliferation and migration. Experimental and Molecular Pathology, 72, 150-160.
  3. Sho, E., Komatsu, M., Sho, M., Nanjo, H., Singh, T.M., Xu, C., Masuda, H.and Zarins, C.K.(2003) High flow drives vascular endothelial cell proliferation during flow-induced arterial remodeling associated with the expression of vascular endothelial growth factors. Experimental and Molecular Pathology, 75, 1-11.
  4. Kato, T., Sato, T., Kurokawa, T., Nanjo, H., Asanuma, Y. and Koyama, K.(2003) Efficacy of continuous infusion of prostaglandin E1 through the superior mesenteric artery against ischemic liver cell necrosis after hepatic artery occlusion. Transplantation, 76, 1340-1345.

社会環境医学講座

感染制御学分野（微生物学講座）
  1. Lee, J.B., Youn, S.J. Nakagomi, T., Park, S.Y. , Kim, T.J., Song, C.S. , Jang, H.K., Kim, B.S. and Nakagomi, O. （2003） Isolation, Serologic and Molecular Characterization of the First G3 Caprine Rotavirus. Arch. Virol., 148,643-657.
  2. Nakagomi, T., Tsunemitsu, H., Imagawa, H. and Nakagomi, O.（2003） Genomic RNA constellation of recently emerging serotype G14 equine rotavirus strains in Japan that is highly homologous with prototype G3 and G14 strains previously identified in the United States of America. Arch. Virol., 148, 925-935.
  3. Laird, A., Gentsch, J., Nakagomi, T., Nakagomi, O.　and Glass, R.I. （2003） Characterization of serotype G9 strains isolated in the United States and India 1993 to 2001. J. Clin. Microbiol., 41, 3100-3111.

生体防御
  1. Honda, K., Sakaguchi, S., Nakajima, C., Watanabe, A., Yanai, H., Matsumoto, M., Ohteki, T., Kaisho, T., Takaoka, A., Akira, S., Seya, T., and Taniguchi, T., (2003)Selective contribution of IFN-α/β signaling to the maturation of dendritic cells induced by double-stranded RNA or viral infection. Proc. Natl. Acad. Sci. USA. 100(19):10872-10877
  2. Holmberg, K., Mariathasan, S., Ohteki, T., Ohashi, P.S., and Gascoigne, N.R.J., (2003)TCR binding kinetics measured with MHC class I tetramers reveal a positive selecting peptide with relatively high affinity for TCR. J. Immunol. 171: 2427-2434
  3. Yamauchi, T., Kamon, J., Ito, Y., Tsuchida, A., Yokomizo, T., Kita, S., Sugiyama, T., Miyagishi, M., Hara, K., Tsunoda, M., Murakami, K., Ohteki, T., Uchida, S., Takekawa, S., Waki, H., Tsuno, N.H., Shibata, Y., Terauchi, Y., Froguel, P., Tobe, K., Koyasu, S., Taira, K., Kitamura, T., Shimizu, T., Nagai, R., and Kadowaki, T., (2003)Cloning of adiponectin receptors that mediated antidiabetic metabolic effects. Nature 423 (No.6941): 762-769
  4. Saibil, S.D., Ohteki, T., White, F.M., Luscher, M., Zakarian, A., Elford, A., Shabanowitz, J., Nishina, H., Hugo, P., Penninger, J., Barber, B., Hunt, D.F., and Ohashi, P.S., (2003)Weak agonist self-peptides promote selection and tuning of virus-specific T cells. Eur. J. Immunol. 33:685-696
  5. Suzuki, H., Matsuda, S., Terauchi, Y., Fujiwara, M., Ohteki, T., Asano, T., Behrens, T.W., Kouro, T., Takatsu, K., Kadowaki, T., and Koyasu, S., (2003) PI3K and Btk differentially regulate B cell antigen receptor mediated signal transduction. Nat. Immunol. 4:280-286

内科学講座

消化器内科学分野・神経内科学分野, 第一内科 （内科学第一講座）
  1. Miura,K., Nagai,H., Ueno, Y. et al (2003) Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. Biochem. Biophys. Res. Commun.,311,415-423.
  2. Nakamura,C., Otaka,Ｍ.,Odashima,M., Jin,M., Konishi,N., Horikawa,Y., Matsuhashi,T. and Watanabe,S.（2003） Rolipram, a specific type IV phosphodiesterase inhibitor, ameliorates indomethacin-induced gastric mucosal injury in rats.Pathophsysiology,9,195-200.

呼吸器内科学分野・循環器内科学分野, 第二内科 （内科学第二講座）
  1. Saito, T., Miura, H., Kimura, Y., Watanabe, H., Nakagomi, A., Tamura, Y., Hasegawa, H., Kibira, S. and Miura, M.（２００３） Reduction of ST Elevation in Repeated Coronary Occlusion Model Depends on Both Altered Metabolic Response and Conduction Properties. Int. J. Cardiol., 92,219-227.
  2. Terata, Y., Saito, T., Fujiwara, Y., Hasegawa, H., Miura, H., Watanabe, H., Chiba, Y., Kibira, S. and Miura, M.（２００３） Pitavastatin Inhibits Upregulation of Intermediate Conductance Calcium-activated Potassium Channels and Coronary Arteriolar Remodeling Induced by Long-term Blockade of Nitric Oxide Synthesis. Pharmacology, 68,169-176.
  3. Iino, K., Watanabe, H., Saito, T., Kibira, S., Iijima, T. and Miura, M.（２００３） TNF-a Rapidly Antagonizes the b-adrenergic Responses of the Chloride Current in Guinea-pig Ventricular Myocytes. Circulation Journal, 67, 347-353.
  4. 鈴木智人（２００３）虚血性心臓交感神経障害におけるNa+チャンネルとNa+/Ca2+交感機構の関与.秋田医学30(2),135-146.

外科学講座

呼吸器外科学分野 , 第二外科 （外科学第二講座）
  1. Motoyama, S., Saito S., Minamiya, Y., Saito, R., Nakamura, M., Okuyama, M., Imano, H .and Ogawa, J.（2003）Methylprednisolone inhibits low flow hypoxia-induced mitochondrial dysfunction in isolated perfused rat liver．Crit. Care Med., 31,1468-1474.
  2. Motoyama, S., Saito, S., Saito, R., Minamiya, Y., Nakamura, M., Okuyama, M., Imano, H. and Ogawa, J. （2003）Hydrogen peroxide-dependent declines in Bcl-2 induces apoptosis in hypoxic liver．J. Surg. Res., 110, 211-216.

神経運動器学講座

脳神経外科学分野, 脳神経外科 （脳神経外科学講座）
  1. Tamura, S.， Kinouchi, H.， Izaki, K.， Okubo, A.， Sugawara, T.， Kunizuka, H. and Mizoi, K.（2003）Induction of heat shock protein 40 and grpE mRNAs following transient focal cerebral ischemia in the rat．Brain Res., 960，277-281．

精神科学分野, 神経科精神科 （精神科学講座）
  1. Masuda, Y., Ohnuma, S., Kawagoe, M. and Sugiyama, T. (2003) A Glycoside of Nicotina tabacum Affects Mouse Doperminergic Behavior. Methods Find Exp. Clin. Pharmacol.,25, 41-43.
  2. Masuda, Y., Suzuki, M., Takemura, T., Sugawara, J., Guo, N., Liu, Y., Kawarada, Y., Shimizu, T.and Sugiyama, T. (2003) Pharmacological Mechanism in Slip-Down Behavior of Mice. Tohoku J. Exp. Med.,201,23-27.

整形外科学分野, 整形外科 （整形外科学講座）
  1. Suzuki, K., Miyakoshi, N., Tsuchida, T., Kasukawa, Y., Sato, K. and Itoi, E.（2003）Effects of combined treatment of insulin and human parathyroid hormone（1-34）on cancellous bone mass and structure in streptozotocin-induced diabetic rats. Bone, 33(1), 108-114.

感覚器学講座

耳鼻咽喉科・頭頸部外科学分野, 耳鼻咽喉科 （耳鼻咽喉科学講座）
  1. Kimura, H.,Kwatani,M.,Ito,E. and Ishikawa,K(2003)Effects of Pituitary Adenylate Cyclase-Activating Polypeptide on facial Nerve Recovery in the Guinea Pig.Laryngoscope,113,1000-1006.

眼科学分野, 眼科 (眼科学講座)
  1. Chen, W. , Ishikawa, M. , Yamaki, K. and Sakuragi, S.(2003) Wistar rat palpebral conjunctiva contains more slow-cycling stem cells that have larger 　 proliferative capacity , Jpn .J . Ophthalmology, 47,119-128.
  2. 山口雅彦 , 後町清子, 山木邦比古（2003）Vogt -小柳-原田（原田）病患者病巣局所から樹立したT細胞株の解析. 秋田医学 30 ,1-11.

生殖発達医学講座

小児科学分野, 小児科 （小児科学講座）
  1. Kikuchi, W., Arai, H., Ishida, A., Takahashi, Y. and Takada, G.. (2003) Distal pulmonary cell proliferation is associated with the expression of EIIIA+ fibronectin in the developing rat lung. Exp. Lung Res., 29, 135-147.

産婦人科学分野 , 産科婦人科 （産科婦人科学講座）
  1. Kawamura,K., Sato,N., Fukuda,J., Kodama,H., Kumagai,J., Tanikawa,H., Murata,M. and Tanaka, T.（2003）The role of leptin during the development of mouse preimplantation embryos.Mol. Cell Endocrinol.,202(1-2),185-189.
  2. Kawamura,K.,Sato,N.,Fukuda,J.,Kodama,H.,Kumagai,J.,Tanikawa,H.,Nakamura,A., Honda,Y.,Sato,T. and Tanaka T.（2003）Ghrelin inhibits the development of mouse preimplantation embryos in vitro. Endocrinology,144(6),2623-2633.
  3. Kawamura,K.,Sato,N.,Fukuda,J.,Kodama,H.,Kumagai,J.,Tanikawa,H.,Shimizu,Y. and Tanaka,T.（2003）Survivin acts as an antiapoptotic factor during the development of mouse preimplantation embryos.Dev. Biol.,256(2),331-41.
  4. Sato,N.,Kawamura,K.,Fukuda,J.,Honda,Y.,Sato,T.,Tanikawa,H.,Kodama,H.and Tanaka,T（2003）Expression of LDL receptor and uptake of LDL in mouse preimplantation embryos. Molecular and Cellular Endocrinology,202,191-194.
  5. 谷川秀郎，河村和弘，本田陽子，佐藤敏治，佐藤直樹，福田淳，児玉英也，田中俊誠（2003）マウス着床前期胚におけるHtrA2/Omiの発現とアポトーシスおよびフラグメンテーションへの関与に関する研究．秋田医学30（2），99-109．

歯科口腔外科
  1. Nakata, A., Kameda, T., Nagai, H., Ikegami, K., Duan, Y., Terada, K. and Sugiyama, T. (2003) Establishment and characterization of a spontaneously immortalized mouse ameloblast-lineage cell line. Biochemistry Biophysics Research Communication, 308, 834-839.
  2. .Miura, K.,Nagai. H.,Ueno, Y. et al.(2003) Epimorphin is involved in differentiation of rat hepatic stem-like cells through cell-cell contact. Biochem. Biophys. Res. Commun.,311, 415-423.

保健学科
  1. 櫛引美代子、吉崎克明、田中俊誠：ラット子宮収縮に対する芍薬甘草湯、当帰芍薬散、温経湯の作用の違い、産婦人科漢方研究のあゆみ20：130-134、診断と治療社、東京、2003