Rodent Surgery Chemical (Cold) Disinfectant/Sterilant Agents Summary Work Surface Sanitation Work surfaces are wiped down with the following agents and allowed to air dry. Sodium Hypochlorite (1:20 dilution of Bleach in water) Chlorine Dioxide (Alcide, Clidox) Roccal D (1:200 dilution in water) Chlorhexidine (Nolvasan solution 1:40 dilution in water) Chemical (Cold) Instrument/Implant Sterilization Instruments must be rinsed with sterile saline or water to remove sterilant before use. Prior to surgery (to kill spores and vegetative cells) Note:Alcohol is not a sterilant and is not acceptable for this purpose. Agent Required Contact Time Ethylene Oxide gas (see text) Glutaraldehydes (Cidex, Sporicidin) 10 hours Between animals (to kill vegetative cells) Glutaraldehyde (Cidex) 10 minutes Zephiran 20 minutes Chlorhexidine (Nolvasan Solution) 20minutes Alcohol (70% isopropyl) 20minutes Chlorine Dioxide (Alcide, Clidox) 3minutes (caution: corrosive to metals) Preoperative Skin Preparation Note: Alcohol alone is not acceptable for this purpose. Povidone-Iodine scrub (betadine) 3 minutes scrub/contact time. Alcohol (70% isopropyl) wipe/rinse to remove iodine scrub. Follow with application of povidone-iodine solution for residual activity. Chlorhexidine scrub (Nolvasan Scrub, Hibiclens) 3 minutes scrub/contact time. Water wipe/rinse to remove excess chlorhexidine scrub. Surgical Attire Sterile Surgical Gloves Laboratory Coat or Scrub Top Surgical Mask
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