APPENDIX I
Rodent Surgery
Chemical (Cold) Disinfectant/Sterilant
Agents Summary
Work Surface Sanitation
Work surfaces are wiped down with the following agents and allowed to air dry.
Sodium Hypochlorite (1:20 dilution of Bleach in water)
Chlorine Dioxide (Alcide, Clidox)
Roccal D (1:200 dilution in water)
Chlorhexidine (Nolvasan solution 1:40 dilution in water)
Chemical (Cold) Instrument/Implant Sterilization
Instruments must be rinsed with sterile saline or water to remove
sterilant before use.
Prior to surgery (to kill spores and vegetative cells)
Note:Alcohol is not a sterilant and is not acceptable for this purpose.
Agent
Required Contact Time
Ethylene Oxide gas (see text)
Glutaraldehydes (Cidex, Sporicidin) 10 hours
Between animals (to kill vegetative cells)
Glutaraldehyde (Cidex) 10 minutes
Zephiran 20
minutes
Chlorhexidine (Nolvasan Solution) 20minutes
Alcohol (70% isopropyl) 20minutes
Chlorine Dioxide (Alcide, Clidox) 3minutes (caution:
corrosive to metals)
Preoperative Skin Preparation
Note: Alcohol alone is not acceptable for this purpose.
Povidone-Iodine scrub (betadine) 3 minutes scrub/contact time.
Alcohol (70% isopropyl) wipe/rinse to remove iodine scrub.
Follow with application of povidone-iodine
solution for residual activity.
Chlorhexidine scrub (Nolvasan Scrub, Hibiclens) 3 minutes
scrub/contact time.
Water wipe/rinse to remove excess chlorhexidine scrub.
Surgical Attire
Sterile Surgical Gloves
Laboratory Coat or Scrub Top
Surgical Mask
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