Brain emulsion from dogs infected with rabies (street virus) was inoculated into the brains of animals.

It was confirmed that monkeys and rabbits developed the disease experimentally.

By repeated passage through rabbits, the incubation period was shortened to six days (fixed virus).

The fixed virus was attenuated to produce a vaccine.

It was confirmed that dogs vaccinated against rabies did not develop the disease even after inoculation with the street virus.

Since the incubation period in humans is long, it was expected that vaccination would still be effective even after being bitten.

July 1885    Vaccination of Joseph Meister (9 years old).

Jean-Baptiste Jupille (15 years old)